Faecalibacterium prausnitzii is Gram-positive, mesophilic, rod-shaped, anaerobic and is one of the most abundant and important commensal bacteria of the human gut microbiota.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBST-036FG
Product Information
Product Overview
Faecalibacterium prausnitzii is Gram-positive, mesophilic, rod-shaped, anaerobic and is one of the most abundant and important commensal bacteria of the human gut microbiota.
Target
Faecalibacterium
Genus
Faecalibacterium
Application
Study and research
Culture Conditions
37°C; Anaerobic
Risk Group
2
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Faecalibacterium is a genus of bacteria. Its sole known species, Faecalibacterium prausnitzii is a Gram-positive, mesophilic, rod-shaped, anaerobic and is one of the most abundant and important commensal bacteria of the human gut microbiota. Faecalibacterium prausnitzii is an extremely oxygen sensitive; EOS bacterium and is difficult to cultivate even in anaerobic conditions and widely distributed in the Gastrointestinal Tract; GIT of other mammals such pigs, mice and calves as well as poultry, and the insect cockroach. Changes in the abundance of fecal Clostridium leptum group, and in particular Faecalibacterium prausnitzii, have been extensively described in different human intestinal and metabolic diseases. Faecalibacterium prausnitzii is a butyrate producer and has demonstrated anti-inflammatory effects in vitro and in vivo using a mouse colitis model making it a key member of the microbiota that may contribute to intestinal homeostasis. Thus, modulation of Faecalibacterium prausnitzii abundance, for example using prebiotics and/or probiotics and/or formulations that permit survival through the upper part of the intestinal tract might have prophylactic or therapeutic applications in human health.
Alternative Names
Faecalibacterium prausnitzii; 27766
FAQs Customer Reviews Related Products
How should Faecalibacterium prausnitzii be stored before use in experiments?
Faecalibacterium prausnitzii should be stored at -80°C to maintain its viability. Aliquoting the culture is highly recommended to avoid multiple freeze-thaw cycles, which can degrade the bacteria's effectiveness. Proper storage conditions are essential to ensure the stability and activity of the bacterial strain.
What is the recommended inoculum size for Faecalibacterium prausnitzii in laboratory cultures?
The recommended inoculum size for Faecalibacterium prausnitzii in laboratory cultures is approximately 2.5 × 10^9 CFU per 100 µL. This concentration is effective in achieving the desired colonization and metabolic activity, facilitating the study of its effects on the gut microbiome and associated health benefits.
Which medium is suitable for culturing Faecalibacterium prausnitzii?
Faecalibacterium prausnitzii is typically cultured in LYHBHI medium (brain heart extract, yeast extract, blood crystal) supplemented with maltose, cellobiose, and cysteine. This medium supports the specific nutritional needs of the bacterium, promoting optimal growth and activity.
How long does it take for Faecalibacterium prausnitzii to reach log phase growth?
Under optimal anaerobic conditions at 37°C, Faecalibacterium prausnitzii typically reaches log phase growth within 24 hours. Monitoring the growth curve is crucial to determine the appropriate time for harvesting cells for downstream applications.
What are the steps involved in preparing Faecalibacterium prausnitzii for in vivo studies?
Preparation for in vivo studies involves growing the culture to the desired phase, harvesting the cells via centrifugation, and resuspending them in a suitable carrier, such as phosphate-buffered saline (PBS). The prepared bacterial solution is then administered to the experimental subjects, ensuring consistent dosing and reliability in research protocols.
Superior Microbiome Modulation
Utilizing Faecalibacterium prausnitzii; 27766 from Creative Biolabs has markedly enhanced our gut microbiome modulation experiments, providing robust and reliable results compared to other brands.
Reliable Results in Metabolic Studies
The application of Faecalibacterium prausnitzii; 27766 in metabolic disorder research has yielded consistently reliable data, making it a superior choice for our laboratory needs.
Enhanced Anti-inflammatory Properties
Research using Faecalibacterium prausnitzii; 27766 demonstrated enhanced anti-inflammatory properties, proving invaluable in our studies on chronic inflammatory diseases.
Outstanding Consistency in Results Faecalibacterium prausnitzii; 27766 from Creative Biolabs provided outstanding consistency in results during our clinical trials, a quality unmatched by other suppliers.
Optimal for Microbiota Studies Faecalibacterium prausnitzii; 27766 has proven optimal for our microbiota studies, delivering high-quality and reproducible results essential for advancing our research.
Click the button below to contact us or submit your feedback about this product.
