This probe provides specific detection of Bacillus cereus, helping to distinguish it from other closely related bacilli. It is used in food safety and soil microbiology research to visualize vegetative cells and spores.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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Product Information
Product Overview
This probe provides specific detection of Bacillus cereus, helping to distinguish it from other closely related bacilli. It is used in food safety and soil microbiology research to visualize vegetative cells and spores.
Target
Microbial FISH Probes
Target Organism
Bacillus cereus
Taxonomic Level
Species
In Silico Coverage
The single-probe coverage rate is 91.31%, and the dual-probe coverage rate is 97.27%.
Probe Types
1. Standard DNA Oligonucleotide Probes 2. Double Labeling Probes
Probe Design Tool
Database used for design (e.g., SILVA TestProbe, RDP, or ProbeBase). https://www.arb-silva.de/documentation/release-1381/
Target Region
16S rRNA
Fluorophore
Cyanine 3, FITC, TR-equivalent or Red Fluorophore, Green Fluorophore.
Purity
≥85% (HPLC purified)
Size
30T/50T
Form
Lyophilized powder or stabilized liquid (in TE buffer).
Concentration
Typically supplied at 50 pmol/μL or 100 pmol/μL (Customizable).
Shipping
Shipped at ambient temperature (lyophilized) or on blue ice (liquid).
Storage
Recommended long-term storage at -20°C in the dark.
Light Sensitivity
Store in the dark. Minimize exposure to ambient light during experiments.
Specificity
Validated against the SILVA/RDP databases.
Documentation Included
1, Certificate of Analysis (COA) 2. Safety Data Sheet (SDS) 3. A standard Hybridization Protocol.
Applications
1. Strain Verification: Confirming the presence and viability of specific probiotic strains in fermented dairy or supplements. 2. Contamination Screening: Rapidly identifying "spoilage" organisms or pathogens in production lines. 3. Metabolic Activity Assessment: Using rRNA-targeted FISH to determine if the bacteria in a product are metabolically active or dormant.
Regulation Status
For research use only (RUO)
Product Features
1. High Taxonomic Specificity: Engineered using advanced thermodynamic modeling to ensure exclusive binding to the target 16S or 23S rRNA sequences with zero cross-reactivity to non-target species. 2. Superior Signal-to-Noise Ratio: Optimized probe chemistry and high-intensity fluorophore conjugation provide bright, distinct signals even in samples with high background autofluorescence. 3. Culture-Independent Detection: Directly detects and quantifies organisms in their natural state, bypassing the biases and time requirements associated with traditional cultivation methods. 4. Broad Sample Compatibility: Fully validated for use across a diverse range of sample types, including FFPE tissue sections, fresh clinical smears, environmental filters, and complex biofilms. 5. Dual-Labeling Capability: Compatible with multi-color FISH protocols, enabling the simultaneous visualization and spatial mapping of multiple different species in a single sample.
