Reliable Probiotic Anti-Obesity Activity Assay to Advance Your Microbiome Therapeutic

Looking to speed up your search for probiotics that fight obesity? Tired of slow progress and struggling to find the right bugs and figure out how they work? Creative Biolabs' Probiotic Anti-Obesity Assays can help you move things along faster. We use smart lab tests and animal studies to get you solid info and point you to the probiotics that show potential. Request a Quote

In Vitro & In Vivo Probiotic Anti-Obesity Activity Assays - Your CRO Solutions

Workflow

In Vitro Assays

Evaluate the impact of probiotics or their components on the cellular and biochemical processes related to obesity.

• Assessment of Lipid Metabolism
  
  • Inhibition of Lipase Activity.
  • Adipocyte Differentiation Assays.
  • Lipogenesis and Lipolysis Gene Expression: Analyzing the expression levels of genes treated with probiotics.
  • Quantification of Intracellular Triglycerides.
  • Fatty Acid Binding Assays: Assessing the capacity of probiotics to bind to fatty acids in vitro.
• Evaluation of Gut Microbiota Modulation
  
  • Antimicrobial Activity Assays.
  • Prebiotic Effects.
  • Production of SCFAs: Measuring the production of SCFAs by the probiotic strain during fermentation.
  • Bile Salt Hydrolase (BSH) Activity: Assessing the probiotic's ability to produce BSH.
• Assessment of Gut Barrier Function
  
  • Adhesion to Intestinal Epithelial Cells: Evaluating the ability of the probiotic strain to adhere to intestinal cell lines (e.g., Caco-2).
  • Improvement of Tight Junction Integrity: Assessing if probiotics can strengthen the tight junctions between intestinal cells, potentially reducing "leaky gut" and associated low-grade inflammation. (Trans-epithelial electrical resistance (TEER) assays.)
• Evaluation of Anti-inflammatory and Other Metabolic Effects
  
  • Cytokine Production Assays.
  • Glucagon-Like Peptide-1 (GLP-1) Secretion Assays.
  • Antioxidant Activity Assays.
  • Cholesterol-Lowering Activity: In vitro assays to determine if probiotics can directly bind or metabolize cholesterol.

In Vivo Assays (Animal Models)

Typically rodents (mice or rats) are fed a high-fat diet, to evaluate the anti-obesity effects of probiotics in a complex biological system.

• Body Weight and Fat Mass Measurement
  
  • Regular monitoring of body weight gain.
  • Measurement of fat mass at the end of the study.
• Metabolic Parameters Assessment
  
  • Food Intake and Energy Expenditure.
  • Glucose Tolerance Tests (GTT) and Insulin Tolerance Tests (ITT).
  • Lipid Profile Analysis.
  • Liver Function Tests.
• Gut Microbiota Analysis
  
  • 16S rRNA Gene Sequencing.
  • Quantitative PCR (qPCR).
  • Metagenomics.
  • Metatranscriptomics.
• Inflammatory Markers and Adipokine Levels
  
  • Measuring circulating levels of pro-inflammatory cytokines and anti-inflammatory cytokines.
  • Assessing the levels of adipokines.
• Histological Analysis
  
  • Examining tissue samples under a microscope to assess adipocyte size, inflammation, and other morphological changes.
• Fecal Metabolite Analysis
  
  • Analyzing the composition of fecal metabolites, including SCFAs.

Additional Services

• Probiotic Strain Characterization.
• Custom Assay Development.

Deliverables

Turnaround Time

The typical timeframe for this service ranges from 8 to 16 weeks, depending on the specific assays required, the number of probiotic strains being evaluated, and the complexity of the in vivo studies.

Applications

Advantages

• Comprehensive Approach.
• Customized Study Design.
• Experienced Team.
• High-Quality Data.
• Fast Turnaround Times.
• Published Data: Our methods adhere to industry standards, ensuring data integrity.

Experience our advantages - Contact us today.

Creative Biolabs is committed to providing cutting-edge solutions for your probiotic research needs. Our probiotic anti-obesity testing service provides a comprehensive method to evaluate the potential of probiotic strains in controlling obesity, and can also flexibly customize your solutions at any stage of the project. Please feel free to contact us for more details.

FAQs

Customer Reviews

• "The comprehensive in vitro screening provided by Creative Biolabs significantly streamlined our strain selection process. The data was clear, and the turnaround time was impressive." - J**n Kim.
• "Using Creative Biolabs' in vivo studies, we were able to demonstrate the efficacy of our probiotic candidate in a relevant animal model. This data was crucial for our regulatory submission." - M**a Silva.
• "Creative Biolabs' detailed mechanism of action studies provided valuable insights into how our probiotic strain exerts its anti-obesity effects. This information will guide our future product development efforts." - D**g Patel.

For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.