Accelerate Probiotic Research: Comprehensive Anti-inflammatory Assay Service

Drawing upon our scientific expertise and commitment to advancing knowledge, Creative Biolabs offers a comprehensive array of assay services precisely engineered for the rigorous assessment of probiotic strains' anti-inflammatory attributes. Our integrated platform encompasses initial in vitro analyses and extends to thorough in vivo validation, furnishing the dependable data crucial for your research endeavors.Request a Quote

In Vitro & In Vivo Anti-inflammatory Assays for Probiotics

Workflow

Initial Characterization of Probiotic Strains

• Materials
  
  • Probiotic strains: Viable or lyophilized format.
  • Strain information: Detailed information including species, subspecies, and any specific cultivation protocols relevant to the strains.
  • Objectives: Clearly defined anti-inflammatory targets or pathways of specific interest.
• Taxonomy and Strain Identification
  
  • 16S rRNA gene sequencing: Strain identification.
  • Whole Genome Sequencing (WGS): Provides a comprehensive genetic profile.
  • MALDI-TOF MS: Rapid identification based on protein profiles.
• Basic Probiotic Properties
  
  • Gastrointestinal Stress Tolerance Testing: Determine the survival rate under different digestive fluid conditions.
  • In Vitro Adhesion assays: Measures the binding efficiency of probiotics to human intestinal epithelial cell lines (e.g., Caco-2, HT-29).
  • Growth and Metabolomics Analysis: Understand the growth curve of probiotic strains and the production of important metabolites (such as SCFAs).
  • Pathogen Growth Inhibition Assays: Evaluate the ability of probiotics to hinder the proliferation of pathogenic bacteria.
  • Safety Assessment: Evaluating for the presence of virulence factors, antibiotic resistance genes, and the production of biogenic amines

In Vitro Anti-inflammatory Assays

• Cytokine Production Assays
  
  • ELISA: Under inflammatory conditions, measure the production of pro-inflammatory and anti-inflammatory cytokines in immune cells.
  • Multiplex Cytokine Assays
• Immune Cell Modulation Assays
  
  • Flow Cytometry: Analyze the influence of probiotics on the immune cell population.
  • Cell Proliferation Assays: Evaluate the impact of probiotics on the growth and division of immune cells.
• Investigating Inflammatory Pathways
  
  • Protein Analysis: Western Blot.
  • Gene Expression Profiling: RT-qPCR for inflammatory mediators.
• Assessing Oxidative Stress and Reactive Oxygen Species (ROS)
• Characterizing Neutrophil Function
• Evaluating Epithelial Barrier Integrity
  Evaluate the effects of probiotics on intestinal epithelial permeability and the expression of tight junction proteins.

In Vivo Anti-inflammatory Assays (Animal Studies)

• Clinical scoring: Monitoring disease symptoms and severity.
• Histological analysis: Examining tissue samples for signs of inflammation and damage.
• Measurement of inflammatory markers in tissues and serum: Cytokines, chemokines, etc.
• Analysis of immune cell infiltration: Assessing the presence of immune cells in inflamed tissues.
• Gut microbiota analysis: Evaluating the impact of probiotics on the overall gut microbial composition.

Data Analysis and Reporting

Following data acquisition from these assays, we employ stringent statistical analyses to ensure the robustness of our findings. A comprehensive report subsequently documents the experimental design, presents the obtained results, and offers a detailed interpretation of the probiotic's observed anti-inflammatory effects.

Deliverables

Turnaround Time

The standard duration for this service typically spans 8 to 16 weeks. This timeframe is contingent upon the specific assays selected, the intricacy of the in vivo models employed, and the number of probiotic strains under evaluation.

Applications

Why Us?

• Comprehensive Approach
• Customizable Solutions
• Expertise and Experience
• High-Quality Data
• Fast Turnaround Time

Experience the Creative Biolabs' Advantage - Get a Quote Today

Our dedication at Creative Biolabs centers on delivering top-tier Probiotic Anti-inflammatory Assay Services, aiming to propel your research and development forward. Our experienced scientific cadre is eager to engage in collaborative partnerships on your project. Please feel free to contact us for more comprehensive information or to explore your specific research demands.

FAQs

Customer Reviews

• "The detailed cytokine profiling provided by Creative Biolabs' Probiotic Anti-inflammatory Assay Services was crucial in identifying the most effective strain for our gut health supplement. The data was clear, concise, and highly reproducible." - J**n S**h.
• "Creative Biolabs' expertise in assay development helped us to establish a robust and reliable method for assessing the anti-inflammatory activity of probiotics in our specific application. Their support and guidance were invaluable." - L**a R**.

For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.