Live Biotherapeutics Drug Discovery Service for Dental Caries
Overview
Dental caries, commonly known as tooth decay, is considered to be the most common oral pathology. Its pathogenesis is very complex, and it is currently believed that the pathogenesis of dental caries is related to ecological stress caused by microbial niche imbalance. It has been observed that dental caries is not caused by specific bacteria, but by a group of bacteria that share common physiological characteristics. Two bacteria were associated with tooth decay; Streptococcus mutans and Lactobacillus, both of which can produce acid during sugar fermentative metabolism, can easily spread in all directions in the oral cavity when these bacteria produce acid in plaque on the tooth surface. This process usually takes months to years to develop into cavities, the endpoint of the disease process of dental caries. Caries can be prevented in two ways: 1) directly by targeting potential pathogens, and 2) indirectly by stopping the "ecological pressure" of responsible pathogen selection. Products containing probiotics have been explored this year as possible alternatives to oral health treatments.
Probiotics for Dental Caries
Probiotics can adhere to oral tissues, prevent the adhesion/colonization/proliferation of caries pathogens and the formation of pathogenic biofilms, produce cell adhesion inhibitors and antibacterial drugs, and consume nutrients before caries pathogens are used. Therefore, probiotics have been proposed as an adjunct to dental caries treatment strategies. Bacteria of different genera can be used as probiotics, with the genera Lactobacillus and Bifidobacterium being most commonly used as probiotic products. Recently, based on in vitro and in vivo experiments, Weissella cibaria, previously classified under the genus Lactobacillus, was demonstrated as a novel probiotic strain to prevent dental caries and significantly inhibit biofilm formation in S. mutans. Probiotic strains used to prevent dental caries have shown promising results, even if only a few studies have shown clear clinical outcomes.
Anti-Caries Effect of Various Probiotics as Per Their Mechanism of Action
Probiotics may have both preventive and therapeutic effects. Their mechanisms of action include adhesion, co-aggregation, growth inhibition, bacteriocin production, and immune regulation. The ability of Lactobacillus to copolymerize helps to prevent the colonization of bacteria that cause dental caries and plaque formation.
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Improved intestinal barrier integrity and upregulation of mucin production
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It participates in the binding of oral microorganisms to proteins (biofilm formation) and plays a role in plaque formation and its complex ecosystem by competing and interfering with the attachment of bacteria to bacteria.
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Producing bacteriocins or other products, has antagonistic effects on pathogenic bacteria, thereby killing or inhibiting the growth of pathogenic bacteria.
Fig.1 Proposed mechanisms of local effects on microorganisms by probiotic lactobacilli.1
Our Services for Dental Caries Research
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Research Articles
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Available Services
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Probiotic effects on multispecies biofilm composition, architecture, and caries activity in vitro.2
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Effect of probiotics on dental caries and periodontal pathogens.3
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Probiotic Lactobacillus sp. inhibits growth, biofilm formation, and gene expression of caries-inducing Streptococcus mutans.4
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The detection of probiotics by in vitro and in vivo preclinical models shows promise in the treatment of periodontal diseases. Creative Biolabs has extensive project experience in the field of live biotherapeutics development and probiotics research and can be very flexible in providing customized solutions to meet customer requirements. Please do not hesitate to contact us, our experts will warmly communicate with you every time so that you have a clear understanding of our services.
References
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Hasslöf, Pamela. Probiotic Lactobacilli in the context of dental caries as a biofilm-mediated disease. Diss. Umeå universitet, 2013.
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Chen, Zhihui, et al. "Probiotic effects on multispecies biofilm composition, architecture, and caries activity in vitro." Microorganisms 8.9 (2020): 1272.
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Routh, Subhajit, et al. "Effect of Probiotics on Dental Caries and Periodontal Pathogens: An: In Vitro: Study." Journal of Orofacial Sciences 11.1 (2019): 49-54.
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Wasfi, Reham, et al. "Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans." Journal of cellular and molecular medicine 22.3 (2018): 1972-1983.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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