Prevotella sp. was isolated from human feces. Its cells are Gram-negative rods that are anaerobic and nonspore-forming.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information | |
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Product Overview | Prevotella sp. was isolated from human feces. Its cells are Gram-negative rods that are anaerobic and nonspore-forming. |
Target | Prevotella |
Genus | Prevotella |
Application | Study and research |
Culture Conditions | 37°C; Anaerobic |
Source | Human feces |
Risk Group | 2 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Prevotella is a genus of anaerobic Gram-negative bacteria of the Bacteroidetes phylum, which also includes the clinically important genera Bacteroides and Porphyromonas. Prevotella strains are classically considered commensal bacteria due to their extensive presence in the healthy human body and their rare involvement in infections. Prevotella spp. are members of the oral, vaginal, and gut microbiota and are often recovered from anaerobic infections of the respiratory tract. Prevotella spp. predominate in periodontal disease and periodontal abscesses. Regarding Prevotella, studies focused on the associations between the diverse species, genome and habitats with dietary patterns, health and disease. Evidence revealed beneficial effects of some Prevotella strains in the gut such as improving CVD risk factor profile and glucose metabolism. Most studies reported that vegetarians harbour higher numbers of Prevotella species as well as a high Prevotella/Bacteroides ratio compared with non-vegetarians; omnivores. Nevertheless, there is a need for more studies in humans to ascertain a causal and potential disease-triggering role for Prevotella and more in-depth metagenomic studies are needed in order to reveal the health- or disease-modulating properties. |
Alternative Names | Prevotella sp. |
Prevotella sp. should be stored at -80°C in a cryoprotectant solution, such as 15% glycerol, to maintain viability. For best results, thaw the culture quickly and transfer it immediately to anaerobic media to ensure optimal recovery and growth conditions.
Culturing Prevotella sp. requires anaerobic conditions at 37°C. Use appropriate anaerobic media like Brain Heart Infusion broth or agar supplemented with hemin and vitamin K1. Strict anaerobic conditions are essential to maintain the viability and proper growth of this Gram-negative, nonspore-forming bacterium.
Isolation of Prevotella sp. from complex samples such as fecal matter involves homogenizing the sample, serial dilution, and plating on selective media like Wilkins-Chalgren agar. The plates are then incubated anaerobically at 37°C for 48-72 hours. Colonies can be identified based on morphology and confirmed using molecular methods such as PCR.
Assays to study the effects of Prevotella sp. on host health include evaluating gut barrier integrity using transepithelial electrical resistance (TEER) measurements, cytokine profiling for immune response evaluation, and molecular techniques such as qPCR and 16S rRNA sequencing for microbial profiling. These methods help assess the impact of Prevotella sp. on gut health and disease mechanisms.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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