Clostridium acetobutylicum is a Gram-positive bacillus. It requires anaerobic conditions in order to grow in its vegetative state. In its vegetative states, it is motile via flagella across is entire surface.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBST-066FG |
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Product Information | |
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Product Overview | Clostridium acetobutylicum is a Gram-positive bacillus. It requires anaerobic conditions in order to grow in its vegetative state. In its vegetative states, it is motile via flagella across is entire surface. |
Target | Clostridium |
Genus | Clostridium |
Application | Study and research |
Type Strain | No |
Culture Medium | Blood agar |
Culture Conditions | 37°C; Anaerobic |
Characteristics | Gram-negative thallus is rod-shaped with motility. The colony is round, rough surface, raised, edge is basically neat, white. Liquid culture has acetone butanol, butyric acid taste, there are settled matter. Gelatin can be liquefied, no pigment. It ferments glucose, sucrose and starch. Catalase positive, milk reaction does not produce acid, coagulation, peptone, incomplete reduction. The highest growth temperature is 39°C, the lowest growth temperature is 32°C. |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Clostridium is a genus of rod-shaped, usually Gram-positive bacteria, members of which are found in soil, water, and the intestinal tracts of humans and other animals. The majority of species are obligate anaerobes. As the predominant bacteria in gut, Clostridium species exert lots of benefits to body health via interacting with intestine directly or indirectly. Clostridium species are potent candidates to alleviate dysfunctions and disorders in intestine, they have been reported to attenuate inflammation and allergic diseases effectively owing to their distinctive biological activities. Their cellular components and metabolites, like butyrate, secondary bile acids and indolepropionic acid, play a probiotic role primarily through energizing intestinal epithelial cells, strengthening intestinal barrier and interacting with immune system. In view of their salutary performances, Clostridium species have a huge potential as probiotics. |
Alternative Names | Clostridium acetobutylicum; 8008 |
The efficiency of fermentation can be enhanced by optimizing the medium composition, pH, and temperature. Additionally, integrating in situ product recovery techniques, such as adsorption with resins, can significantly increase butanol yields by reducing inhibitory by-products.
For long-term storage, Clostridium acetobutylicum should be kept at -80°C in a cryoprotectant medium containing glycerol. This ensures cell viability during storage and subsequent revival.
Thaw frozen Clostridium acetobutylicum stocks quickly at 37°C and immediately transfer to an anaerobic medium. Incubate under anaerobic conditions until visible growth is observed, then proceed with scaling up the culture as required for the experiment.
Solvent production capabilities can be assessed using gas chromatography to measure concentrations of butanol, acetone, and ethanol in the fermentation broth. Additionally, metabolic flux analysis and enzyme activity assays can provide insights into the metabolic pathways involved.
Genetic modifications, such as CRISPR/Cas9 and other metabolic engineering tools, can be used to enhance solvent production, improve stress tolerance, and manipulate metabolic pathways. These modifications can lead to higher yields and more efficient fermentation processes.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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