Oxalobacter formigenes as Next Generation Probiotics
Background O. formigenes for PH Disease Services Products FAQs Resources
Introduction of O. formigenes
The bacterium Oxalobacter formigenes (O. formigenes) is currently being developed as a potential treatment for primary hyperoxaluria in patients at different stages of renal function. As a leader in live biotherapeutic products (LBPs) research, Creative Biolabs can provide flexible solutions for your LBP development projects. You can start our cooperation and rely on us.
Introduction of O. formigenes
One of the most efficient oxalate-degrading organisms is O. formigenes, a Gram-negative, non-pathogenic, anaerobic commensal bacterium, naturally occurring in the colon of humans and other vertebrates and exclusively relying on oxalate to formate conversion as its energy source. The presence of oxalate-powered bacteria, isolated from the faeces of patients without kidney stones, has been associated with reduced oxalate excretion in the urine. It increases the active transcellular secretion of oxalate from the blood to the gastrointestinal tract through interaction with transporter proteins, possibly from the SLC26 family.
Fig.1 (A) Electron micrographs of O. formigenes strain OxWR1, (B) Gram stain of O. formigenes strain HOxBLS. 1
O. formigenes for Primary Hyperoxaluria (PH) Disease
The effect of O. formigenes on nephrocalcinosiswas studied in ethylene glycol rat models simulating hepatic oxalateoverproduction in PH. Plasma and urine oxalic acid levels, calcium oxalate crystalluria, urinary volume, fluid intake, and serum creatinine were monitored during the study. Ethylene glycol intake induced pronounced hyperoxaluria, hyperoxaluria, calcium oxalate crystalluria, and nephrocalcinosis. Concomitant O. formigenes treatment partially prevented the ethylene glycol-induced increase in plasma oxalate and completely prevented nephrocalcinosis. These results showed a beneficial effect of O. formigenes treatment on ethylene glycol-induced hyperoxaluria and nephrocalcinosis, and thus support a possible beneficial effect of O. formigenes in PH.
Fig.2 Overview of the possible gut-kidney axis in nephrolithiasis, with O. formigenes at the center of a bacterial network involved in oxalate degradation and protecting against the onset of hyperoxaluria.2
What Services Can We Provide for O. formigenes at Creative Biolabs?
O. formigenes Related Products at Creative Biolabs
We supply a variety of O. formigenesstrainsof different preservation numbersas below. If you need other strains, please contact us.
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Customized strain culture supernatant. (e.g.: for animal research)
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Customized strain lyophilized powder containing certain CFU. (e.g.: for animal research)
Treatment with O. formigenes represents a novel therapeutic approachto potentially prevent further CaOx deposition. At Creative Biolabs, we have the expertise to optimize each stage to ensure you get your desired outcome. Please feel free to contact us for further discussion of O. formigenes projects.
FAQs
Can O. formigenes supplementation help in conditions other than calcium oxalate kidney stones?
While the primary research focus has been on preventing kidney stones, some studies suggest that O. formigenes may help manage other conditions associated with oxalate metabolism, such as certain forms of inflammation and cardiovascular issues linked to oxalate.
Are there any side effects associated with O. formigenes treatments?
Currently, there are no major side effects reported with the use of O. formigenes in a therapeutic setting. However, as with any probiotic, individual responses can vary, and its use should be monitored in clinical settings.
What research is being done to enhance the efficacy of O. formigenes as a probiotic?
Research is ongoing to identify genetic markers and environmental conditions that maximize the probiotic effects of O. formigenes. This includes studies on gene expression involved in oxalate degradation and strategies to improve bacterial survival and colonization in the gut.
What are the advantages of using Creative Biolabs' O. formigenes services for research?
Creative Biolabs specializes in offering tailored O. formigenes strains and services that enhance research flexibility and outcome reliability. Additionally, we provide extensive support, including detailed characterization and quality assurance of all bacterial strains, which ensures high standards and reproducibility in research projects.
Resources
References
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Daniel,S.L.; et al. Forty Years of Oxalobacter formigenes, a Gutsy Oxalate-Degrading Specialist. Applied and Environmental Microbiology. 2021, 87(18): e00544-21.
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Ticinesi,A.; et al. Gut microbiome and kidney stone disease: Not just an Oxalobacter story. Kidney International.2019, 96(1): 25-27.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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