| LBGF-0126-GF1 |
Escherichia coli Nissle 1917-miRFP713 |
This strain expresses a near-infrared (NIR) fluorescent protein, allowing for deep-tissue imaging in living animals. Because NIR light can penetrate biological tissues better than visible light, this strain is a premier tool for non-invasive in vivo tracking. |
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| LBGF-0126-GF4 |
Escherichia coli Nissle 1917-mCitrine |
A yellow-fluorescent strain that utilizes an improved version of the Yellow Fluorescent Protein (YFP). It is engineered to be less sensitive to environmental factors like pH and salt, which are common in the gut. |
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| LBGF-0126-GF5 |
Escherichia coli Nissle 1917-mOrange2 |
This strain produces a bright orange fluorescence, providing a unique spectral signature between yellow and red. It is optimized for photostability, meaning it can be imaged for longer periods without fading. |
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| LBGF-0126-GF7 |
Escherichia coli Nissle 1917-EGFP |
The classic "industry standard" for green fluorescence, this strain expresses the Enhanced Green Fluorescent Protein. It is the most common tool for basic localization and quantification of bacteria in biological assays. |
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| LBGF-0126-GF8 |
Escherichia coli Nissle 1917-luxCDABE |
This strain is bioluminescent and generates its own light through a chemical reaction. It requires no external light source to be seen, making it ideal for "dark" imaging deep within the body. |
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| LBGF-0126-GF10 |
Escherichia coli Nissle 1917ΔtolAΔnlpI |
This is a structurally compromised strain engineered for maximum Outer Membrane Vesicle (OMV) production. By deleting two critical components of the cell wall and membrane tethering system, the strain becomes hyper-vesiculating. |
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| LBGF-0126-GF11 |
Escherichia coli Nissle 1917ΔtolRΔmlaE |
This strain is a specialized "hyper-vesiculating" platform designed for the mass production of Outer Membrane Vesicles (OMVs). By disrupting both the structural anchoring of the cell envelope and the lipid transport machinery, the strain sheds high concentrations of vesicles into the culture medium. |
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| LBGF-0126-GF12 |
Escherichia coli Nissle 1917ΔNIPIΔmsbB |
This is a "detoxified" probiotic host engineered to minimize systemic inflammatory responses during therapeutic use. It features a modified lipopolysaccharide (LPS) structure that significantly reduces its potency as an endotoxin while maintaining bacterial viability. |
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| LBGF-0126-GF13 |
Escherichia coli Nissle 1917ΔompT |
This strain serves as an optimized factory for the production of secreted recombinant proteins. It is engineered to remove a specific "gatekeeper" protease that frequently degrades high-value proteins as they pass through the outer membrane. |
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| LBGF-0126-GF14 |
Escherichia coli Nissle 1917ΔLon |
This modification creates a "protease-free" internal environment to enhance the stability of heterologous proteins synthesized within the cytoplasm. It prevents the bacterium's natural quality-control systems from destroying proteins that the cell perceives as foreign or misfolded. |
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| LBGF-0126-GF15 |
Escherichia coli Nissle 1917Δlpp |
This strain is a "leaky" phenotype engineered to bypass the traditional need for mechanical cell lysis during protein harvesting. By removing a major structural anchor, the cell wall becomes porous, allowing internal products to diffuse into the surrounding broth. |
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| LBGF-0126-GF16 |
Escherichia coli Nissle 1917ΔendA |
This strain is optimized for the propagation and extraction of high-quality plasmid DNA for gene therapy or laboratory research. It eliminates a common contaminant that degrades DNA during the purification process. |
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| LBGF-0126-GF17 |
Escherichia coli Nissle 1917ΔclbA |
This modification is a "safety-first" engineering step aimed at removing the genotoxic potential of the native Nissle 1917 strain. It ensures that the probiotic can be used long-term in the human gut without the risk of causing DNA damage to the intestinal lining. |
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| LBGF-0126-GF18 |
Escherichia coli Nissle 1917ΔTolRAΔmsbB |
This is a sophisticated "safe-delivery" strain that combines hyper-vesiculation with detoxified endotoxins. It is designed to produce a large volume of OMVs that are safe for systemic administration in humans. |
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| LBGF-0126-GF19 |
Escherichia coli Nissle 1917ΔnlpI |
This strain is engineered to exhibit increased outer membrane instability, leading to the spontaneous release of outer membrane vesicles (OMVs). By removing a key regulator of cell wall peptidoglycan metabolism, the bacterium becomes a more efficient vehicle for secreting vesicle-packaged cargo. |
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| LBGF-0126-GF20 |
Escherichia coli Nissle 1917ΔtolR |
This strain is a classic "hyper-blebbing" model characterized by a significant loss of connection between the inner and outer bacterial membranes. It is primarily used in research to study membrane dynamics or to harvest large quantities of bacterial surface components.Escherichia coli Nissle 1917 tolR Mutant Are Highly Heterogeneous and Show Reduced Capacity for Epithelial Cell Interaction and Entry |
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| LBGF-0126-GF21 |
Escherichia coli Nissle 1917ΔtonB |
This modification creates an "iron-starved" phenotype by disrupting the energy-coupling mechanism required for nutrient uptake. It is often used as a safety "kill-switch" or to study the metabolic limits of the probiotic in the gut. |
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| LBGF-0126-GF22 |
Escherichia coli Nissle 1917ΔiroN |
This strain is specifically impaired in its ability to utilize salmochelin, a specialized iron-binding molecule. This modification targets one of the "stealth" iron acquisition systems that bacteria use to evade the host's nutritional immunity. |
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| LBGF-0126-GF23 |
Escherichia coli Nissle 1917ΔiutA |
This engineered strain is deficient in the uptake of aerobactin, a high-affinity siderophore often associated with bacterial fitness in low-iron environments. It is a targeted modification to reduce the "competitiveness" of the probiotic. |
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| LBGF-0126-GF24 |
Escherichia coli Nissle 1917ΔfyuA |
This modification removes the receptor for yersiniabactin, an iron-scavenging system often found on the "High Pathogenicity Island" (HPI) of many E. coli strains. Its deletion further attenuates the strain's ability to survive in the host. |
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| LBGF-0126-GF25 |
Escherichia coli Nissle 1917ΔchuA |
This strain is engineered to be unable to utilize heme (the iron-containing part of hemoglobin) as an iron source. This is a critical modification for strains intended to operate in the gastrointestinal tract where blood may be present. |
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| LBGF-0126-GF26 |
Escherichia coli Nissle 1917ΔldhAΔadhE |
This strain is a metabolically engineered host designed to redirect carbon flux away from organic acid production and toward target pathways like succinate or ethanol. By blocking the primary fermentation pathways that consume NADH, the bacteria are forced to utilize alternative metabolic routes. |
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| LBGF-0126-GF27 |
Escherichia coli Nissle 1917ΔmchBCDEFΔmcmIA |
This strain is engineered to be deficient in the production and immunity of Microcin H47, a potent antibacterial peptide. It is used to study the role of bacteriocins in gut colonization or to create a "blank slate" for the insertion of new antimicrobial tools. |
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| LBGF-0126-GF28 |
Escherichia coli Nissle 1917ΔKpsM |
This modification targets the K5 capsule biosynthesis, resulting in a "naked" or non-encapsulated bacterial surface. This strain is significantly more susceptible to environmental stress and host immune recognition compared to the wild-type. |
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| LBGF-0126-GF29 |
Escherichia coli Nissle 1917ΔtolC |
This strain is engineered to be highly sensitive to antibiotics and metabolic waste by disrupting the cell's primary "exhaust pipe." It lacks the ability to pump out toxic substances, making it a fragile but highly controllable host. |
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| LBGF-0126-GF30 |
Escherichia coli Nissle 1917(DE3) Δrnc |
This is a specialized strain designed for the stabilization of double-stranded RNA (dsRNA) and T7-based protein expression. The "(DE3)" indicates it carries the T7 RNA polymerase, while the knockout prevents the degradation of complex RNA structures. |
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| LBGF-0126-GF31 |
Escherichia coli Nissle 1917ΔtolAΔtolBΔtolC |
This triple-knockout strain is a "maximally unstable" model designed for extreme membrane permeability and total secretion failure. It combines the loss of structural integrity (TolAB) with the loss of the primary export channel (TolC). |
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| LBGF-0126-GF32 |
Escherichia coli Nissle 1917-lux-tyr |
This is a bioluminescent reporter strain engineered to be "self-glowing" and metabolically dependent on specific amino acids. It allows researchers to track the bacteria in real-time within a living host (in vivo) without the need for external excitation light. |
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| LBGF-0126-GF33 |
Escherichia coli Nissle 1917-pVDL9.3 |
This strain carries the specialized pVDL9.3 plasmid, which is a high-stability vector designed for the long-term expression of therapeutic genes. It is engineered to remain inside the bacteria even in the absence of antibiotic selection pressure, which is critical for use in the human gut. |
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| LBGF-0126-GF34 |
Escherichia coli Nissle 1917-sfGFP |
This strain is labeled with Superfolder Green Fluorescent Protein (sfGFP), a highly robust version of GFP that folds rapidly and resists denaturation. It is used as a high-visibility marker for tracking individual bacteria under a microscope or via flow cytometry. |
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| LBGF-0126-GF35 |
Escherichia coli Nissle 1917-ClyA-Hy |
This is a therapeutic delivery strain engineered to secrete ClyA-fusion proteins using the bacteria's natural pore-forming protein as a "shuttle." It is specifically designed to export therapeutic payloads directly into the surrounding environment, such as a tumor microenvironment. |
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| LBGF-0126-GF36 |
Escherichia coli Nissle 1917-GFPuv |
This strain expresses a variant of Green Fluorescent Protein optimized for excitation by UV light rather than blue light. It serves as a specialized diagnostic tool for identifying bacterial colonies in complex environmental or biological samples. |
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| LBGF-0126-GF37 |
Escherichia coli Nissle 1917-pglB |
This strain is a "bio-glycosylation" factory engineered to attach complex sugars to proteins. By carrying the pglB gene, the bacteria gain the ability to create glycoconjugate vaccines, which are highly effective at training the immune system. |
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| LBGF-0126-GF40 |
Escherichia coli Nissle 1917Δlpp::A5 |
This platform strain features a "knock-in/knock-out" modification where the major structural lipoprotein (Lpp) is removed and replaced with an A5 |
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| LBGF-0126-GF43 |
Escherichia coli Nissle 1917-pelB-ANXA5 |
This strain utilizes the pelB leader sequence to direct the Annexin A5 protein to the periplasmic space or the extracellular environment. This ensures that the targeting protein is properly folded and positioned to interact with host cells. |
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| LBGF-0126-GF44 |
Escherichia coli Nissle 1917Δlpp::A5-RFP |
This is a dual-purpose strain that combines theΔlpp structural modification with Red Fluorescent Protein. It allows researchers to track a "leaky" or "surface-display" strain in real-time using fluorescence. |
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| LBGF-0126-GF45 |
Escherichia coli Nissle 1917Δlpp::A5-LuxCDABE |
This strain combines theΔlpp::A5 surface display platform with the LuxCDABE bioluminescence operon. It creates a "self-lighting" version of the leaky structural mutant, eliminating the need for external excitation light for imaging. |
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| LBGF-0126-GF47 |
Escherichia coli Nissle 1917Δlpp::A5-aTN |
This strain uses theΔlpp::A5 surface display platform to present anti-TNF-alpha agents directly on the bacterial surface. By tethering the drug to the outside of the cell, the bacteria can "mop up" inflammatory cytokines as they swim through the gut. |
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| LBGF-0126-GF48 |
Escherichia coli Nissle 1917-BCD-BUT |
This is a metabolically "supercharged" strain engineered to produce high levels of Butyrate, a short-chain fatty acid. Butyrate is a primary energy source for colon cells and is essential for maintaining gut barrier integrity. |
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| LBGF-0126-GF2 |
Escherichia coli Nissle 1917-mTagBFP2 |
A blue-fluorescent reporter strain designed for high-brightness labeling at the short-wavelength end of the spectrum. It provides a distinct color channel for complex experiments involving multiple bacterial types. |
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| LBGF-0126-GF3 |
Escherichia coli Nissle 1917-mTurquoise2 |
This strain expresses a bright cyan fluorescent protein, serving as a high-performance marker. It fills the "cyan" gap in the imaging spectrum with exceptional clarity. |
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| LBGF-0126-GF38 |
Escherichia coli Nissle 1917-Sj16 |
This is a specialized therapeutic strain engineered to express and secrete the Sj16 protein, a peptide derived from a parasite (Schistosoma japonicum). This strain is designed to act as a "living immunomodulator" to treat inflammatory diseases. |
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| LBGF-0126-GF39 |
Escherichia coli Nissle 1917-VHB |
This strain is engineered to express Vitreoscilla hemoglobin (VHB), which enhances the bacterium's ability to survive and grow in low-oxygen (hypoxic) environments. It acts as an "oxygen scavenger," allowing the probiotic to remain metabolically active in deep tissues or dense biofilms. |
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| LBGF-0126-GF41 |
Escherichia coli Nissle 1917-RFP |
This strain is a standard reporter model expressing Red Fluorescent Protein (RFP). It provides a bright, stable red signal that is easily distinguishable from the green autofluorescence often found in plant matter or animal tissues. |
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| LBGF-0126-GF42 |
Escherichia coli Nissle 1917-ANXA5 |
This strain is engineered to express Annexin A5 (ANXA5), a protein that has a high affinity for phosphatidylserine, a marker of cell death (apoptosis). It is designed to act as a "living sensor" that can home in on damaged or dying tissues. |
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| LBGF-0126-GF46 |
Escherichia coli Nissle 1917-aTN |
This strain is engineered to produce an anti-tumor necrosis factor (aTN) molecule, such as a neutralizing peptide. It acts as a localized "pharmacy" in the gut to reduce inflammation. |
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