| Product Overview |
Targeting Lactobacillus acidophilus, a prominent probiotic, this probe is used to monitor its presence in fermented foods and the gut. It allows for the verification of colonization in clinical research. |
| Target |
Microbial FISH Probes |
| Target Organism |
Lactobacillus acidophilus |
| Taxonomic Level |
Species |
| In Silico Coverage |
The theoretical coverage of this probe is optimized during the design phase using the most current rRNA databases (e.g., SILVA, RDP). However, final in-silico coverage and specificity parameters are confirmed upon completion of the specific probe sequence synthesis and preliminary testing. |
| Probe Types |
1. Standard DNA Oligonucleotide Probes
2. Double Labeling Probes |
| Probe Design Tool |
Database used for design (e.g., SILVA TestProbe, RDP, or ProbeBase).
https://www.arb-silva.de/documentation/release-1381/ |
| Target Region |
16S RNA |
| Fluorophore |
Cyanine 3, FITC, TR-equivalent or Red Fluorophore, Green Fluorophore. |
| Purity |
≥85% (HPLC purified) |
| Size |
30T/50T |
| Form |
Lyophilized powder or stabilized liquid (in TE buffer). |
| Concentration |
Typically supplied at 50 pmol/μL or 100 pmol/μL (Customizable). |
| Shipping |
Shipped at ambient temperature (lyophilized) or on blue ice (liquid). |
| Storage |
Recommended long-term storage at -20°C in the dark. |
| Light Sensitivity |
Store in the dark. Minimize exposure to ambient light during experiments. |
| Specificity |
Validated against the SILVA/RDP databases. |
| Documentation Included |
1, Certificate of Analysis (COA)
2. Safety Data Sheet (SDS)
3. A standard Hybridization Protocol. |
| Applications |
Oral & Vaginal Health Studies
1. Periodontal Research: Analyzing the complexity of subgingival plaque and the interactions between "Red Complex" pathogens.
2. Vaginal Flora Balancing: Assessing the ratio of protective Lactobacillus species versus dysbiotic anaerobic bacteria (e.g., Gardnerella).
3. Biofilm Penetration: Testing the efficacy of oral rinses or topical treatments in penetrating dense microbial matrices. |
| Regulation Status |
For research use only (RUO) |
| Product Features |
1. High Taxonomic Specificity: Engineered using advanced thermodynamic modeling to ensure exclusive binding to the target 16S or 23S rRNA sequences with zero cross-reactivity to non-target species.
2. Superior Signal-to-Noise Ratio: Optimized probe chemistry and high-intensity fluorophore conjugation provide bright, distinct signals even in samples with high background autofluorescence.
3. Culture-Independent Detection: Directly detects and quantifies organisms in their natural state, bypassing the biases and time requirements associated with traditional cultivation methods.
4. Broad Sample Compatibility: Fully validated for use across a diverse range of sample types, including FFPE tissue sections, fresh clinical smears, environmental filters, and complex biofilms.
5. Dual-Labeling Capability: Compatible with multi-color FISH protocols, enabling the simultaneous visualization and spatial mapping of multiple different species in a single sample. |
